Characterization of Matrigel interfaces during defined human embryonic stem cell culture

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Characterization of Matrigel interfaces during defined human embryonic stem cell culture.

Differences in attachment, proliferation, and differentiation were measured for human embryonic stem (hES) cells cultured on various substrata coated with Matrigel, a blend of extracellular matrix proteins derived from murine tumor cells. The authors observed that hES cells attach and grow poorly on Matrigel adsorbed onto polystyrene, while they proliferate when exposed to Matrigel adsorbed ont...

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Human embryonic stem (hES) cells are pluripotent, capable of differentiating into any cell type of the body, and therefore have the ability to provide insights into mechanisms of human development and disease, as well as to provide a potentially unlimited supply of cells for cell-based therapy and diagnostics. Knowledge of the adhesion receptors that hES cells employ to engage extracellular mat...

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Defined culture conditions of human embryonic stem cells.

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ژورنال

عنوان ژورنال: Biointerphases

سال: 2009

ISSN: 1934-8630,1559-4106

DOI: 10.1116/1.3274061